Coding

Part:BBa_J428061:Design

Designed by: Vinoo Selvarajah   Group: 2021 Engineering Committee   (2022-06-07)


Cas_variants_hLwCas13a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2830
    Illegal BglII site found at 1286
    Illegal BglII site found at 1496
    Illegal BglII site found at 1763
    Illegal BglII site found at 2528
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2830
    Illegal PstI site found at 2851
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added stop codon (taa).


Source

Source = Addgene 90097.

References